Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Titration of the Histone H3.3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 1-30 from human H3F3A was used as the immunogen for this Histone H3.3 antibody.

Aliquot the Histone H3.3 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.