Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress,HSF1 acquires DNA-binding ability and localizes to nuclear stress granules.SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.

Titration of the HSF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 278-309 from the human protein (sumoylation site) was used as the immunogen for this HSF1 antibody.

Aliquot the HSF1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.