Integrin-binding sialoprotein, also known as Bone sialoprotein and BSP, is a major structural protein of the bone matrix. Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

The stated application concentrations are suggested starting amounts.Titration of the Ibsp antibody may be required due to differences in protocols and secondary/substrate sensitivity.

An amino acid sequence from the C-terminus of mouse Bone sialoprotein (SYYKGHGYEGYEGQNYYYHQ) was used as the immunogen for this Ibsp antibody.

After reconstitution, the Ibsp antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.