IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts. [UniProt]

Titration of the IGHM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 103-131 from the human protein was used as the immunogen for this IGHM antibody.

Aliquot the IGHM antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.