NF-kB is sequestered in the cytoplasm by IkBa, unable to translocate to the nucleus.IkB is part of the canonical pathway for NF-kB activation.IKKb is part of the IkB kinase complex, along with a second catalytic subunit, IKKa, and a regulatory subunit, IKKg.Along with IKKa, IKKb phosphorylates IkBa leading to its degradation and release of inhibition of NF-kB. The RELA subunit of active NF-kB is then free to enter the nucleus and initiate a number of signaling cascades.

Provided assay concentrations are suggestions only, IKKb antibody titration may be required for optimal results.

A recombinant protein fragment of IKKb was used as the immunogen for this antibody.

Aliquot the IKKb antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

I kappa B kinase beta, IKK2, IKBKB, IMD15, NFKBIKB