Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.
Titration of the ILK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 321-350 from the human protein was used as the immunogen for this ILK antibody.
Aliquot the ILK antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
