After removal of the precursor signal peptide, proinsulinis post-translationally cleaved into three peptides: the B chainand A chain peptides, which are covalently linked via two disulfidebonds to form insulin, and C-peptide. Binding of insulin to theinsulin receptor (INSR) stimulates glucose uptake. A multitude ofmutant alleles with phenotypic effects have been identified. Thereis a read-through gene, INS-IGF2, which overlaps with this gene atthe 5" region and with the IGF2 gene at the 3" region. Alternativesplicing results in multiple transcript variants. [provided byRefSeq].

Titration of the Insulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 35-64 from the human protein was used as the immunogen for this Insulin antibody.

Aliquot the Insulin antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.