ITGB4, also known as CD104, is a receptor for the laminin proteins. It tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. It was found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells and ZKSCAN3 has been confirmed as the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines.

Titration of the ITGB4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Human partial recombinant protein (AA 28-266) was used as the immunogen for this ITGB4 antibody.

After reconstitution, the ITGB4 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.