May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). [UniProt]

The stated application concentrations are suggested starting points. Titration of the Klotho antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 402-436 from human Klotho was used as the immunogen for the Klotho antibody.

Aliquot the Klotho antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.