XRCC6 (X-Ray Repair, Complementing Defective, In Chinese Hamster, 6), also called Ku70, G22P1 or TLAA, is a protein that in humans, is encoded by the XRCC6 gene. The XRCC6 gene encodesthesubunit p70 of the p70/p80 autoantigen which consists of two proteins of molecular mass of approximately 70,000 and 80,000 daltons that dimerize to form a 10 S DNA-binding complex. XRCC6 and Mre11 are differentially expressed during meiosis. XRCC6 interacts with Baxa, a mediator of mitochondrial-dependent apoptosis. Disruption of both FANCC and XRCC6/Ku70 suppressed sensitivity to crosslinking agents, diminished chromosome breaks, and reversed defective homologous recombination. Ku70 binds directly to free DNA ends, committing them to NHEJ repair. In early meiotic prophase, however, when meiotic recombination is most probably initiated, Mre11 was abundant, whereas Ku70 was not detectable.

The stated application concentrations are suggested starting amounts.Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

An amino acid sequence from the C-terminus of human Ku70 (YPPDYNPEGKVTKRKH) was used as the immunogen for this Ku70 antibody.

After reconstitution, the Ku70 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.