Ku70 is a single stranded DNA-dependent ATP-dependent helicase. It has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3"-5" direction. Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 432-461 from the human protein was used as the immunogen for this Ku70 antibody.

Aliquot the Ku70 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.