L1CAM is an axonal glycoproteinbelonging to the immunoglobulin supergene family. The ectodomain,consisting of several immunoglobulin-like domains andfibronectin-like repeats (type III), is linked via a singletransmembrane sequence to a conserved cytoplasmic domain. This celladhesion molecule plays an important role in nervous systemdevelopment, including neuronal migration and differentiation.Mutations in the gene cause three X-linked neurological syndromesknown by the acronym CRASH (corpus callosum hypoplasia,retardation, aphasia, spastic paraplegia and hydrocephalus).Alternative splicing of a neuron-specific exon is thought to befunctionally relevant.

Titration of the L1CAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. Cleavage by ADAM10 results in 180 and 40 kDa bands while cleavage by Plasmin results in 140 and 80 kDa bands.

A portion of amino acids 1154-1182 from the human protein was used as the immunogen for this L1CAM antibody.

Aliquot the L1CAM antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.