LCK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3"-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LCK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.

Titration of the LCK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 23-52 from the human protein was used as the immunogen for this LCK antibody.

Aliquot the LCK antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.