Double-stranded RNA viruses are recognized in a celltype-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 andRIGI. These interactions initiate signalingpathways that differ in their initial steps but converge in theactivation of the protein kinases IKKA and IKKB which activate NFKB, or TBK1 and IKKE (IKBKE; MIM 605048), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB. For the TLR3 pathway, the intermediarymolecule before the pathways converge is the cytoplasmic proteinTRIF. For RIGI, the intermediary protein ismitochondria-bound IPS1.

Titration of the MAVS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 477-505 from the human protein was used as the immunogen for this MAVS antibody.

Aliquot the MAVS antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.