MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Titration of the Mib1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 13-42 from the human protein was used as the immunogen for this Mib1 antibody.
Aliquot the Mib1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
