MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7 (MAPKK7), JNKK2, PRKMK7, Mek7 and MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al.(2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 / Mek7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. the protein was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al.(2011) concluded that MKK7 / Mek7 exerts its tumor suppressive function through p53.

The stated application concentrations are suggested starting amounts.Titration of the MKK7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

An amino acid sequence from the N-terminus of human MEK7/MKK7 (ARRRIDLNLDISPQRPR) was used as the immunogen for this MKK7 antibody (100% homologous in human, mouse and rat).

After reconstitution, the MKK7 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.