NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 72-101 from the human protein was used as the immunogen for this NRAS antibody.

Aliquot the NRAS antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.