Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5"-UUAGGG-3" repeats more tightly than the telomeric single-stranded DNA 5"-TTAGGG-3" repeats. [UniProt]

Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This Nucleolin antibody was produced from mice immunized with a protein from human NCL.

Aliquot the Nucleolin antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.