Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.

Aliquot the PGK1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.