CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

Titration of the phospho-CRK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This phospho-CRK antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS41 of human CRK.

Aliquot the phospho-CRK antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.