E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. It contains a putative zinc-binding motif and a highly acidic region.PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells.It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
The stated application concentrations are suggested starting amounts.Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat).
After reconstitution, the PIAS1 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.
