Interferon-induced, double-stranded RNA-activated protein kinase (PRKR/PKR) is a serine-threonine kinase.Activation by dsRNAs leads to autophosphorylation of PKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. PRKR, which is involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, activates IKK and NFKB, thereby suppressing apoptosis. Apoptosis induced by live pathogenic gram-positive and gram-negative bacteria requirs both TLR4 and PKR, possibly representing a major mechanism for pathogenic bacteria that use specific virulence factors to avoid detection and destruction by the innate immune system.

Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 11-42 from the human protein was used as the immunogen for this PKR antibody.

Aliquot the PKR antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.