Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Titration of the REST antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 542-570 from the human protein was used as the immunogen for this REST antibody.
Aliquot the REST antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
