Src associated in mitosis 68 kDa protein is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to.

Titration of the SAM68 antibody may be required due to differences in protocols and substrate sensitivity.

A portion of amino acids 259-291 from the human protein was used as the immunogen for the SAM68 antibody.

Aliquot the SAM68 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.