Recognizes a homodimeric protein comprised of 50kDa subunits, identified as Semaphorin-4D, or CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Optimal dilution of the Semaphorin-4D antibody to be determined by the researcher.

PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

Store the Semaphorin-4D antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).