This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Optimal dilution of the TGF-alpha antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.

Store the TGF-alpha antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).