It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.

Amino acids 56-66 (DSGNDIYGNPI) from the human protein were used as the immunogen for this TIMP-2 antibody.

Store the TIMP-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).