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主营:肿瘤,肿瘤生物标志物,细胞凋亡,免疫学,细胞生物学和转录因子
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当前位置: 首页 > 产品中心 > Functional_antibody > NSJ Bio/ZAP-70 Antibody [clone ZTP70-1] (V7156)/100 ug/V7156-100UG
商品详细NSJ Bio/ZAP-70 Antibody [clone ZTP70-1] (V7156)/100 ug/V7156-100UG
NSJ Bio/ZAP-70 Antibody [clone ZTP70-1] (V7156)/100 ug/V7156-100UG
NSJ Bio/ZAP-70 Antibody [clone ZTP70-1] (V7156)/100 ug/V7156-100UG
商品编号: V7156-100UG
品牌: NSJBioreagents
市场价: ¥9980.00
美元价: 7984.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description

Tyrosine-protein kinase ZAP70 is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, it phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. [UniProt]

Application Notes

Titering of the ZAP-70 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the ZAP-70 antibody.

Storage

Store the ZAP-70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

品牌介绍
NSJ Bioreagents已有20多年的历史。 NSJ Bio提供领先的、广泛使用的单克隆抗体以及单克隆和多克隆抗体的所有领域的研究,包括肿瘤,肿瘤生物标志物,细胞凋亡,免疫学,细胞生物学和转录因子。产品提供多种规格,以更好地适应研究人员的测试需求和预算限制。   链接:http://www.nsjbio.com/ 温馨提示:不可用于临床治疗。 风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。